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No new species have been observed
- The Giant Evening Primrose, Oenothera gigas, arose from Lamarck's Evening Primrose O. lamarckiana, through polyploidy, in that the former has twice as many chromosomes as its parents (2N=28 versus 2N=14).
- The Kew Primrose Primula kewensis arose from fertile tetraploid mutants from an otherwise sterile hybrid cross between P. floribunda and P. verticillata.
- The Honeysuckle Maggot Fly, Rhagoletis mendax Ã— zephyria, of North America, is descended from a hybrid between Snowberry Maggot Flies and Blueberry Maggot Flies around 250 years ago, and has been afflicting imported European honeysuckle vines used as ornamental plants in Eastern North America ever since. It was determined to be a hybrid species when scientists recreated new honeysuckle maggot flies by breeding the two parent species in laboratories.
- The mosquito species Culex molestus lives only in the underground of the British city of London, having descended from a population of the species C. pipiens that was stranded there over a century ago. The two species are physically and genetically similar, but can not interbreed, and prefer different prey (the former prefers humans and rodents whereas the latter prefers birds).
- Molecular tests done on the rare desert sunflower species Helianthus anomalis and H. deserticola show that they are descendants of diploid hybrids between the two widespread species, H. annuus and H. petiolaris.
- Domestic sheep are a result of humans using a process known as artificial speciation - selecting existing genetic variants within species or hybridizing different subspecies or breeds to create new species that are more suited to human use.
- The evolution of new species has also been observed in the fossil record numerous times for example the fossil bird Archaeopteryx appears to have evolved from the earlier dinosaur species Compsognathus , and the first species of modern horses Equus stenonis appears to be descended from more primitive horses such as Plesippus.
- Modern cattle are also a result of artificial speciation they are descended from the extinct species of wild cattle Bos primigenius.
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Fallacies Contained in this Claim
- Suppressed Evidence (clear cut contrary evidence is ignored)
- Bloomberg, David, 1993. Observed Instances of Speciation. 
- Stassen, C. et al, 1992-1997. Some More Observed Speciation Events. 
- Kimball, John W., 2001. Speciation. 
- Corliss, William R., 1987. Instances of observed speciation. 
- Wikipedia. Artificial speciation
- Wild Helianthus anomalus and H. deserticola from the Desert Southwest USA: a Potential Source of Stress Genes for Cultivated Sunflower 
- Mark Isaak's page for this claim 
- Anon, 1998 (26 Aug.). London underground source of new insect forms. London Times, 
- Anon, 1998 (Oct.). Science notes: Mutant Culex pipiens mosquitoes in the London Underground. ASTMH Newsletter,  See also
- Anon, 2000. Clocks and alternative lifestyles. Science 289: 1435. See also: Simon et al., 2000. Evolution 54: 1326.
- Beheregaray LB, Sunnucks P, 2001. Fine-scale genetic structure, estuarine colonization and incipient speciation in the marine silverside fish Odontesthes argentinensis. Molecular Ecology 10(12): 2849-2866.
- Brown, Charles W., n.d. Ensatina eschscholtzi Speciation in Progress: A Classic Example of Darwinian Evolution. 
- Fanello C, Petrarca V, et al., 2003. The pyrethroid knock-down resistance gene in the Anopheles gambiae complex in Mali and further indication of incipient speciation within An. gambiae s.s. Insect Mol. Biol. 12(3): 241-245.
- Filchak, Kenneth E., Joseph B. Roethele & Jeffrey L. Feder, 2000. Natural selection and sympatric divergence in the apple maggot Rhagoletis pomonella. Nature 407: 739-742.
- Irwin, Darren E., Staffan Bensch & Trevor D. Price, 2001. Speciation in a ring. Nature 409: 333-337. 
- Korol, A. et al., (2000) Proceedings of the National Academy of Science USA 97: 12637-12642. See also Schneider, C.J., 2000 (Oct. 31). Natural selection and speciation. Proceedings of the National Academy of Science USA 97.
- Lehmann T, Licht M, Elissa N et al, 2003. Population Structure of Anopheles gambiae in Africa. J. Hered. 94(2): 133-147.
- Mayr, E., 1942. Systematics and the Origin of Species, Columbia University Press.
- Nevo, Eviatar, 2000. Mosaic Evolution of Subterranean Mammals: Regression, Progression and Global Convergence. Oxford University Press.
- Van Valen, Leigh M. & Maiorana, Virginia C., 1991. HeLa, a new microbial species. Evolutionary Theory 10: 71-74.
- Wake, David B., 1997. Incipient species formation in salamanders of the Ensatina complex. Proceedings of the National Academy of Science USA 94: 7761-7767.
- Whitehouse, David, 2001 (18 Jan.). Songbird shows how evolution works. BBC News Online, 
- Woodmorappe, 1996. Noah's Ark: A Feasability Study, ICR, El Cajon, CA.
- Evolution hasn't been observed nowadays
- Macroevolution has never been observed
- No new phyla, classes, or orders have appeared